https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Paradigm shift in frog sperm cryopreservation: reduced role for non-penetrating cryoprotectants https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53343 50% for each). Theoretically, it has been suggested that increased NPCP concentration should improve cell survival by increasing the rate and extent of cell dehydration. We suggest, however, that the elevated osmolality in the unfrozen water fraction when 10% sucrose is used may be causing damage to cells via excessive cell shrinkage and solute effects as proposed in the two-factor hypothesis of cryoinjury. We showed this response in sperm across a range of frog species, providing compelling evidence for this hypothesis. We suggest protocol development using the PCP/NPCP ratios demonstrated in our study will be broadly applicable to many amphibian species.]]> Wed 22 Nov 2023 10:19:22 AEDT ]]> Storage and retrieval of spermatozoa for the conservation of threatened reptiles: a lizard model for sperm cryopreservation https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48051 Wed 13 Mar 2024 14:13:07 AEDT ]]> Development of sperm cryopreservation and assisted reproductive technologies for the conservation of threatened Australian tree frogs https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53557 Thu 14 Mar 2024 08:46:39 AEDT ]]>